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The academy-razzing double nude "Venus and Psyche" — in which a masculine-looking brunette leans with an equivocal expression (hatred?
One of Aga's new advertisements shows a hip-looking Sloane Ranger in a black cocktail dress standing in front of a black Aga, with an equivocal expression one commentator here interpreted as "You can't actually believe I would wreck my manicure cleaning this thing, do you?" The ad reads: "Welcome to the New Iron Age".
The academy-razzing double nude "Venus and Psyche" — in which a masculine-looking brunette leans with an equivocal expression (hatred? lust?) over a sumptuous, sleeping blonde — was submitted to the Salon of 1864 and, though in a close call, was also rejected, for indecency.
HER2neu ratio, HER2neu gene analysis by fluorescence in situ hybridization (FISH) compared with a normal control, ratio < 2.0 is normal expression, ratio 2 to 4 is equivocal expression, and ratio >4.0 is gene overexpression.
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Next to immunohistochemistry (IHC) to evaluate HER2 protein overexpression, a second line gene amplification test is generally deemed necessary for cases with equivocal protein expression.
More frequently, one or more of these risk factors are borderline (e.g. G2 tumor; equivocal HER2 expression; borderline HER2-gene status, borderline proliferation index), leading to uncertainty about the tumor behavior, which in turn impairs the ability to reach informed treatment decisions.
Although our fuzzy gene signatures could accurately distinguished grade 1 from grade 3 tumors and separated grade 2 tumors in grade 1 like or grade 3 like, we defined an indecision zone that scored arbitrarily between 48 and 52%, due to the equivocal gene expression grade profile of these patients.
In 5/15 tested cases (including CH34 which had intact PTEN expression), equivocal PTEN methylation specific PCR results were obtained in which only the MSP2 primer set amplified the methylated target at nucleotide −298 upstream in the promoter sequence while the MSP1 primer set targeting nucleotide −984 did not.
The data may also be equivocal because gene expression may change in hybrid genotypes.
Tumors exhibiting equivocal HER-2 expression, denoted as 2+ membranous staining of tumor cells, are confirmed by fluorescence in situ hybridization (FISH) at an outside laboratory.
The implication of amplification-independent HER2 overexpression is that patients with equivocal Her2 protein expression (Her2 score 2+) might be denied clinical anti-HER2 adjuvant therapy (AT).
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