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To assess whether the transcriptional effect was transmitted to protein levels, we calculated the ratio of the protein levels in carbon-limitation to the level in nitrogen-limitation at D = 0.2 h-1. Figure 10(c) shows the distribution of these ratios plotted against the equivalent transcript level ratio.
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Transcripts of two genes were clearly enriched in female antennae compared to male antennae, whereas other genes displayed relatively equivalent transcript levels in antennae of both sexes.
Since multiple transcript and protein variants expressed by the same gene are, by definition, structurally distinct and need not to be functionally equivalent, the concept of gene orthology should be extended to the transcript level in order to describe evolutionary relationships between structurally similar transcript variants.
Sub-clustering of orthologue groups at the transcript level is an important step to more accurately define functionally equivalent orthologue groups.
Within each of these superfamilies, a significant and roughly equivalent number of genes were either up-regulated or down-regulated by NaCl at the transcript level, suggesting dynamic regulation of glycosylation in response to stress.
NRT1.1 regulates CIPK8 and CIPK23 at the transcript level.
Fgf-2 transcript was down-regulated, while Fgf-18 transcript level was strongly up-regulated.
Although many thousands of alternatively spliced transcripts were detected at the mRNA transcript level, the evidences of AS at the protein level are controversial34, 35, 64, 65.
Transcript level expression data quantified using StringTie were stored in GTF (Gene Transfer Format) providing expression levels for both known as well as novel transcripts against mouse reference genome (mm10-Mus_musculus.GRCm38.84.gtf).
Y-axis, relative transcript level compared with rice OsUbi1.
Interestingly, EGTA treatment repressed the transcript level of OsNCX genes.
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