We have observed that the first step towards cell death –as measured either by trypan blue staining or by fluorescence-activated cell sorting (FACS) using propidium iodide that gave equivalent quantifications [46]– is cell rounding and detachment of the monolayer.
The lower limit of quantification, equivalent to the lowest point in the calibration curve, was 0.1 ng/ml; the percentage of samples in which levels were not detectable was 11.8 % overall (15.9 % in phase 1 samples and 5.6 % in phase 2 samples).
The plots in Fig. 3 demonstrate the equivalent
For the real time PCR assays the limit of quantification was equivalent to the virus infectivity cut off, which equates to a 93-fold increase in sensitivity.
On the basis of the equivalent variables, an uncertainty quantification model is established, in which the unified probabilistic information related to the uncertain responses of engineering systems can be computed with the aid of the fuzziness discretization and reconstruction, the belief and plausibility measures analysis, and the interval response analysis.
Subsequent quantification was equivalent between techniques and specimens, and differences between specimen types in several experiments were <0.5 log10 copies/mL.
Because it is unlikely that the relation between toxic responses and the degree of alteration in the biomarker is equivalent at all doses, quantification of risks at low levels is not necessarily more accurate using these biomarkers for extrapolation.
Quantification of mutant-type Ki-ras gene in plasma samples is inappropriate due to the low amount of DNA and the absence of cellular equivalents which allows the quantification of single copy genes as a reference.
Standard biochemical compositional analysis revealed no particular differencesbetween the different maize types and diets, the GM and non-GM maize being classifiedas substantially equivalent, except for transgene DNA quantification.
A simple quantification of the equivalent linear damping ratio, modeling the first fore-aft mode of the tower as a 1-DOF linear damped mass spring system, permits a rough estimation of the contribution to the global damping of the aeroelastic effects.
