Duplicate PCR reactions were set up in a final volume of 25 ul using 10 ng equivalents of input total RNA.
Equivalent amounts of input protein in the cell extracts were verified with the anti-HA antibody (see Materials and methods for details).
There was also no statistically significant shift in the proportion of miRs in each abundance stratum between equivalent combinations of input RNA concentration and cDNA dilution [chi-square test, (χ2 = 3.3, df = 6, p = 0.77)].
An equivalent amount of input DNA was used for NGS as a negative control and acetylated lysine 9 of histone H3 (Ac-H3K9) was included as a positive control for actively transcribed genes.
For equivalent amounts of input tau (Fig. 7A, upper panel), both wild-type and S2A tau formed high molecular weight aggregates in the soluble fraction, whereas in the insoluble fraction the tau protein detected was monomeric (Fig. 7A, lower panel).
Using equivalent amounts of input LCM tissue (1000 laser 'shots') from both CP compartments, the endothelial marker CD31 was significantly enriched in the CP capillary tissue, while the epithelial marker cytokeratin 8 was observed in CP epithelial tissue alone.
We carried out microtubule-binding assays for wild-type, S2A and S11A tau by incubating equivalent amounts of input wild-type or mutant tau (Fig. 7B, upper panel) with paclitaxel-stabilized microtubules.
In his well-known "Chinese nation" thought-experiment, Block (1980b) imagines that the population of China (chosen because its size approximates the number of neurons in a typical human brain) is recruited to duplicate his functional organization for a period of time, receiving the equivalent of sensory input from an artificial body and passing messages back and forth via satellite.
The pulled-down materials and 3% equivalent of the input samples were subjected to SDS-PAGE followed by western blot analysis with anti-PB1, -PB2, -PA, -NP, and -GST (only the pull-down sample) antibodies.
AV454 is run on a subset of the reads (∼ 11k, equivalent to 1%8% 8% of input).
