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In parallel, equivalent extracts were analyzed by SDS PAGE and western blotting.
Equivalent extracts were incubated with 25 µl of guanosine 5′-triphosphate-agarose (Sigma-Aldrich) by rotating for 2 h at 4°C.
No fragment was amplified from equivalent extracts prepared from uninfected plants or with species-specific primer pairs for BMCTV, BSCTV or BCTV (Fig. 1F).
Equivalent extracts were resolved on Native-PAGE 3 12% Bis Tris gradient gels (Invitrogen) and blots were subjected to western blotting with anti-Dnm1, anti-Drp1, anti-Mfn2, anti-OPA1 and anti-LRRK2 antibodies.
In contrast, nuclear extracts from MCF-7TXT9 and MCF-7TXT10 cells exhibited more than threefold higher levels of subunit binding to the NF-κB sequence compared with equivalent extracts from MCF-7CC cells (P < 0.05).
To estimate Smc protein and parS-359 DNA abundance in B. subtilis cells, levels of Smc and parS-359 in extracts of wild-type cells were compared to defined amounts of purified Smc protein and parS-359 DNA spiked into equivalent extracts from cell harboring a tagged version of Smc and a modified parS-359 locus (see panel C ).
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Next, we tested whether the pollen fractions contain candidate allergens by spotting equivalent extract quantities (normalized to total protein) onto nitrocellulose membranes and blotting with human sera pooled from 500 American individuals of diverse age and ethnicity.
Both Orc6 and Sld2, two important S-CDK substrates (Nguyen et al, 2001; Masumoto et al, 2002; Wilmes et al, 2004), migrated more slowly in SDS PAGE in the S-phase extract compared to the equivalent extract made from cells held in G1 phase.
Thus, the S1/P3 pairs of samples had equal volumes of CHO or Balb/MK cell-equivalent extracts, which were also equivalent to the TCE lysates.
The aqueous extraction of salt followed by HCl extraction of ulvan gave higher yields (8.2 ± 1.1% w/w) and purity of ulvan than equivalent Na2C2O4 extracts (4.0 ± 1.0% w/w).
During endpoint assays with papain, a similar trend was observed when treated with 5 mg equivalent Oscillatoria extracts as compared with that of control which showed papain activity as 43.20 ± 3.09 µM tyrosine min−1 (mg protein)−1.
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