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Quality criteria were applied to the genomic data based on per-sample and per-SNP call rates and Hardy Weinberg equilibrium testing.
a P value for Hardy-Weinberg equilibrium testing.
PLINK (version 1.06, http://pngu.mgh.harvard.edu/purcell/plink) [ 47] was used for Hardy-Weinberg equilibrium testing.
For Hardy-Weinberg (HW) equilibrium testing the Online Encyclopedia for Genetic Epidemiology studies (http://www.oege.org) was used.
Open image in new window Fig. 4 XRD patterns of the cement pastes after equilibrium testing at 28 days.
Hardy-Weinberg equilibrium testing was performed using Pearson's goodness-of-fit chi-squared test with one degree of freedom.
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An in-depth discussion on the rationale of the setting applied for the Hardy-Weinberg equilibrium test is provided in Suppl.
The final set SNPs were filtered by keeping those with missing rate ≤10%, MAF ≥5%, SNP calling rate per accession ≥80%, and P value of Hardy-Weinberg equilibrium test ≤0.001.
Kinetic experimental procedures were identical to the equilibrium tests.
The equilibrium tests were carried out at different constant temperatures (5, 15, 25 and 50 °C).
The procedures of kinetic experiments were basically identical to those of equilibrium tests.
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