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For the 1 mm spacing, the device equilibrated to approximately 2% oxygen in about 15 min. Equilibration for the hypoxic chamber was orders of magnitude slower, taking about 3 h to reach approximately 2.5% oxygen which is in agreement with previously published data [27].
After equilibration for 1 h, the polymerization was initiated by adding 109.6 μmol ammonium persulfate (APS).
All measurements were measured at room temperature after equilibration for 10 min.
All measurements were measured at room temperature after equilibration for 10 min. The data were obtained with the average of three measurements.
Here, we characterize sfGFP, and find that this variant exhibits hysteresis as unfolding and refolding equilibrium titration curves are non-coincident even after equilibration for more than eight half-lives as estimated from kinetic unfolding and refolding studies.
However, as discussed above, rapid desorption of 10-5 M Cu from kaolinite after equilibration for 72 hours at pH 10.5 suggests that adsorption is the primary uptake mechanism.
After proper equilibration for both systems a 1 ns production run was carried out using NPT at 298 K and 11.0 bar, the trajectories were collected each 1 ps for analyses.
The animals underwent controlled haemorrhage of 25 ml/kg during 15 min followed by equilibration for 10 min and then bled another 5 ml/kg during 10 min or a sham procedure.
In Fig. 6, the bottom panel shows the S-to-P energy ratio at three receivers with normalized distances of 0.2, 1 and 2. The top panel shows the S-to-P energy ratio in all the space (or global equilibration) for comparison.
After equilibration for 5 min at 37°C, 0.1 ml stimulus was added and light emission recoded continuously.
This was followed by equilibration for 1 ns in the final NPT ensemble before the production phase.
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