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Cells were lysed with a Branson sonifier for a total of 15 min with 3 s bursts at 50% amplitude interspersed with 6 s pauses to equilibrate temperature.
After centrifugation for 15 min at 2,000 x g to attach mitochondria, 450 μl of MAS (containing substrate) was added to each well, and the plate was incubated at 37°C for 8 min to equilibrate temperature.
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The plate was loaded into a Flexstation (Molecular Devices, Wokingham, United Kingdom) and allowed to equilibrate to temperature (37°C).
This uncertainty becomes apparent when comparing measured equilibrated borehole temperatures to modelled temperatures from shallower points.
The reaction was then equilibrated (room temperature, 15 minutes) and neutralised (10 μl of 1 M Tris-HCI and 3 μl of 2 N HCl).
The thermal probes were allowed to equilibrate with skin temperature and the skin temperature was measured and recorded.
Kamenetsky et al. (2004) documented the presence of sodalite and inclusions in olivine containing halide-carbonate mineral assemblages, which equilibrated at temperatures of well above 400 °C.
The samples were left to equilibrate at the temperature of the experiment for 1 h.
The mixtures were then vortexed for 15 s and left to equilibrate at room temperature overnight.
The generator and the water bath were switched off, and vesicles were left to equilibrate at room temperature for 1 h.
The pH 7 samples did not equilibrate at any temperature during the experiment.
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