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The recipient oysters were randomly and equally transferred and reared in five commercial and contrasting grow-out locations.
The different lanes were equally transferred to a PVDF membrane, and then the membrane was processed for immunobloting using specific primary antibody against Akt (polyclonal, Cell signaling), phosphorylated Akt (Ser 473, monoclonal, Cell Signaling), eNOS (polyclonal, BD Biosciences) and phosphorylated eNOS (Ser 1177, polyclonal, Cell Signaling), respectively.
However, this subjectivity should be equally transferred to both types of specimens.
After collection, samples were mixed gently and separated, equally transferred into 2 uniquely labelled clear polypropylene tubes (at least 500 μL of milk per aliquot), and placed in a −80 °C deep freezer.
Briefly, L4 animals were grown on NGM agar plates (2.5 g peptone, 17 g agar, 3 g NaCl per 1 l) seeded with bacterial strain HB101 as animals' food at 20°C for 5 6 days; adults were collected by washing with S-basal buffer [5 g NaCl and 50 ml of 1 m potassium phosphate (pH 6.0) per liter] and equally transferred into two unautoclaved 1.5 ml microcentrifuge tubes.
Similar(55)
Under the medium hard coals and soft coals, the immediate roof can equally transfer the overturn deformation of the main roof to top coals and the support system.
At the end of toxicity and the chemotherapeutic trials, a single blood samples (2.5ml) was collected from each birds from the wing vein or by jugular venipuncture and transferred equally into vacutainer tubes with or without heparin as anticoagulant.
These qualities emerged first on the battlefield – and Roberts's account of the engagements at Toulon and Marengo that propelled him to eminence are gripping – but, most unusually, they transferred equally to the realm of government.
Cells from one well of a six-well plate were then transferred equally into four wells of a 24-well plate and either mock or infected with the indicated virus.
After enough growth, the substrate (1, 1.0 g) dissolved in acetone (20 mL), was transferred equally to all the flasks under sterilized conditions.
Ten ml of fasting venous blood was collected and transferred equally into a heparinized tube and a non-heparinized tube.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com