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Samples are from same gel and brightness/contrast was adjusted equally prior to cropping.
Flesh fly life stages that were individually RNA extracted and combined equally prior to cDNA library synthesis and normalization.
Seven custom multiplexing adapters compatible with Illumina single-end sequencing were used and indexed DNA samples were pooled equally prior to PCR enrichment.
The results demonstrate that inherent differences exist between dyadic and triadic relationships; prior pair-wise interactions were more likely to lead to future pair events and, equally, prior group events lead to future group events.
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Around 20 hr after transfection, cells were lysed, clarified and equally divided prior to incubation with 30 µl of mouse IgG agarose (A0919, Sigma), or 15 µl of mouse anti-HA agarose (ThermoScientific), respectively, for 2 hr at 4°C.
Around 20 hr after transfection, cells were lysed, clarified and equally divided prior to incubation with either 2 μg of mouse IgG control (Santa Cruz), 2 μg of ALK mAb31, or 2 μg of mouse anti-HA (Covance) antibody.
However, it has to be noted that for identification of differential gene expression by array analysis, it is highly preferable that each sample is equally treated prior to hybridisation, as every amplification method has the possibility to introduce transcript-dependent biases, which even increase as the starting amount of RNA decreases (Stirewalt et al, 2004).
Consistent with predictions, hostility and depression correlated equally well with prior bullying victimization but only hostility successfully mediated the relation between prior bullying victimization and subsequent bullying perpetration.
All applications are reviewed equally, regardless of prior connection with Cornell University.
The agencies were equally unprepared linguistically prior to the conflicts in Somalia, the Balkans and, tragically, Afghanistan.
However, equally well the prior awareness state may just play the role of a warning for the agent to be prepared that the action will happen (anyway).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com