For blank control, equal volume of the same buffer used in the final step of protein purification was added.
15% aqueous extract was shaken vigorously in a separating funnel three times with equal volume of the solvent.
Equal volume of the culture broth from Penicillium sp. was extracted with equal volume of butanol in separating funnel.
At predetermined time points, aliquots were withdrawn from the beaker and replaced with equal volume of the media.
5 ml of DPPH solution in 90%% methanol was mixed with an equal volume of the extract and ascorbic acid.
Equal volume of the standard solution were mixed and injected in the HPLC system in volumes of 2, 4, 6, 8 and 10 µl for plotting calibration curves.
When the viscosities of the liquids were similar, inversion occured at roughly equal volume of the phase, with small differences caused by the surface wetting.
In the present investigation, a standardized extract of G. biloba (EGb 761) or an equal volume of the vehicle was administered (100 mg/kg/day) to senescent (20-month) C57BL/6 male mice for up to 82 consecutive days.
The probiotic viability-enhancing effect of each permeate and retentate was assessed with the acid stress assay described above by combining 6 mL of L. rhamnosus HN001 working culture with an equal volume of the ultrafiltered fractions.
Each bottle was thoroughly mixed to have an even distribution of microbes, and then, equal volume of the sample from each bottle of water was taken and mixed together to have a composite sample of the desired volume.
The pH was varied during reduction by adding different amounts of NaOH solution.10 ml of 3 × 10-3(M) trichloroacetic acid solution was added to an equal volume of the same concentration of alkaline CTAB.
