Sentence examples for equal transfer of the from inspiring English sources

To confirm an equal transfer of the proteins to the membranes, stripping of the membranes was performed when indicated using 50 ml of stripping buffer for 30 min in a water bath at 50°C (Kornmann et al, 1999b).

Staining with Ponceau Red was done to confirm equal transfer of protein in all lanes.

Based on the theory of Equal Transfer Process of Transmission Lines (ETPTLs), a new high-speed distance relay scheme is proposed in order to overcome above problem.

α-tubulin antibody was used to control for equal loading and transfer of the samples, as mentioned previously.

Membranes were blocked with 5% milk and then incubated with α-tubulin antibody (B-7 sc-5286, mouse monoclonal 1∶600, Santa Cruz Biotechnology, CA) to control for equal loading and transfer of the samples.

Equivalent amounts of total protein extract from each sample were electrophoretically separated on 10 15% discontinuous acrylamide gels, transferred to pure nitrocellulose membranes (PROTRAN BA85, Schleicher and Schuell, 0.45 µm), and the equal loading and transfer of the samples was monitored by Ponceau staining of the membranes.

Alpha-Tubulin antibody was used to control for equal loading and transfer of the samples.

For BCA assay coefficient of intra- and interassay variations was less than 2.10% and 7.94%, respectively (with mean CV% values of 1.36% and 4.34%, resp .. Equal loading and transfer of the western blot samples were further verified and compared with beta-actin by reversible total protein staining of the nitrocellulose membrane with Ponceau-S reversible membrane staining.

The gel was stained with ethidium bromide to verify equal loading of samples before capillary transfer of the RNA overnight onto Hybond-N nylon membrane (Amersham Biosciences, Little Chalfont, UK).

The maximum measured value equalled a transfer of 284 mL oxygen/min.

After electrotransfer, the nitrocellulose membrane (Protran, Schleicher & Schuell, Dassel, Germany) was stained with Ponceau S (PonS) in order to control complete transfer and equal loading of the lanes.