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Based on the theory of Equal Transfer Process of Transmission Lines (ETPTLs), a new high-speed distance relay scheme is proposed in order to overcome above problem.
Staining with Ponceau Red was done to confirm equal transfer of protein in all lanes.
To check equal loading on the gels and equal transfer, protein loading was visualised on the membranes using Gelcode (Pierce) according to the manufacturer's instructions (data not shown).
To confirm an equal transfer of the proteins to the membranes, stripping of the membranes was performed when indicated using 50 ml of stripping buffer for 30 min in a water bath at 50°C (Kornmann et al, 1999b).
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The measurements show that, for equal transferred charge and identical porous silicon (PSi) layers, the applied current intensity also influences the final Er content.
Membranes were routinely stained with Ponceau red for visualization of proteins and to confirm adequate transfer and equal loading.
Equal loading and adequate transfer to the membranes were verified by staining with Ponceau S (Sigma-Aldrich) and with anti-GAPDH (Sigma-Aldrich) antibody.
Proteins were transferred to nitrocellulose membranes and stained using the MemCode Reversible Protein Staining Kit (Thermo Fisher Scientific) to ensure equal protein transfer.
Similarly, in high frequency region, the nearly identical radii of semicircles also imply the equal charge transfer resistance (R ct).
The thermal resistance is significantly lower for FP than for straight pipe exchangers, due to the lower amount of material consisting the FP for equal heat transfer surface.
From the comparison of vibratory agitation with conventional rotary agitation, it is found that vibratory agitation needs less energy than rotary agitation to attain an equal mass transfer coefficient under these experimental conditions.
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