What happens, actually, is potassium is rushing in here because in this experiment there's an equal concentration of potassium put on both sides of the membrane.
After addition of 2x Laemlli buffer, equal concentration of protein was resolved on 10% SDS-PAGE followed by immunoblotting with anti-Hsp70 and anti-actin antibodies on nitrocellulose membrane.
Equal concentration of denatured proteins were separated on 10% SDS-polyacrylamide gels and were transferred onto polyvinylidene difluoride (PVDF) membranes, blocked by 5% nonfat dry milk for 1 hr, followed by incubating with rabbit polyclonal anti-Ano1 antibody (Abcam ab53213, 1 500 dilution) for overnight.
To examine if this type of ANP has the similar effect on ICaL as ANP 3 28 in our model, equal concentration of ANP1 28 was tested.
Subsequently, CSF samples representing equal concentration of Tf (determined by ELISA) from CJD- and CJD+ cases were incubated with equal counts of 59FeCl3 to radiolabel Tf, dialyzed to remove free 59FeCl3, and fractionated on a native gel (Figure S2 C).
There are equal concentrations of glucose on both sides of the cell.
Despite the six catalysts being prepared through different methods using equal concentrations of Mn supporting on TiO2, the Mn 2p3/2 XPS spectra of Mn/TiO2 samples were conducted and surface atomic ratios were calculated (Fig. 6; Table 2), which evaluates the oxidation state and interaction between Mn species and TiO2 supports.
Cellular GPX activity was calculated based on equal concentrations of protein extracts determined by BCA protein assay kit (Pierce) with BSA as a standard.
The libraries were pooled together two and two in equal concentration for each lane on the flow cell, and sequenced as single reads to 100 bp on an Illumina HiSeq 2000.
Protein concentrations of cell lysates were estimated according to Lowry et al., 1951 [21] and equal concentrations of protein fraction were run on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunobloted with specific antibodies.
