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This was surprising because Tris and HEPES have nearly equal buffer capacities (see Materials and Methods) in our recording conditions (pH 7.8; 4 mM HEPES: 2.0 mM versus 4 mM Tris: 2.1 mM).
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Interestingly, HEPES is more efficient in inhibiting feedback than Tris, whereas they have about equal pH buffer capacity in our experimental conditions (pH 7.8; 4 mM HEPES: 2.0 mM versus 4 mM Tris: 2.1 mM).
Aliquots of 25 μL of organic modifier or detergent at various concentrations were pipetted into a 96-well plate that already contained either 75 μL binding buffer (equal to 0% displacement) or 75 μL 33.3 μM E2 in binding buffer (equal to 100% displacement).
Equal volumes of buffer were added to the same volume of GdnHCl solutions and these mixtures were used as blank.
Negative controls consisted of all of the same reagents but substituting for the target with an equal volume of buffer.
The start of each frame is separated by a fixed number of samples, which is equal to the buffer size.
Five hundred μl of crude culture filtrate was incubated with equal volumes of buffer containing ABTS and laccase activity was measured at 405 nm.
In the latest rendition of its bail-in scheme, TBTF banks are required to keep a buffer equal to 16-20% of their risk-weighted assets in the form of equity or bonds convertible to equity in the event of insolvency.
In order to obtain the Multiple Lamellar Vesicles (MLVs) the dry lipids were hydrated with an equal weight of buffer (5 mM HEPES pH 7.4).
After being heated for 5 min at 95°C in a sample buffer, equal aliquots of the cell lysates were run on a 10% SDS polyacrylamide gel.
Furthermore, the fully folded spectrum obtained experimentally could be weighted and subtracted from the spectrum of MET16 at equal peptide and buffer concentrations to obtain the unfolded spectrum.
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