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The root tip cells and epidermal cells of the control roots were intact while the epidermis peeled off and the root cap destructed in the treated roots (Figure 6a-b, c-d).

The rake is used to "comb" the horny layer (stratum corneum) of the epidermis, knocking off dead cells.

Using a razorblade 0.5 × 0.5 cm pieces were cut out from fresh scale and the epidermis peeled off from the inner side with tweezers.

To assure that most stomata are open before beginning experiments, plants were kept under light for 3 hrs, and the epidermis of N. benthamiana leaves was peeled off and immediately floated on stomata opening buffer (10 mM MES-KOH, 30 mM KCl, pH6.3) for 3 hrs.

Keratinocytes in the outmost stratum corneum (SC) of the epidermis are sloughed off and replaced by newly differentiated cells originating from epidermal stem cells located in the basal layer [3], [4].

On the following day, the epidermis was peeled off the dermis, and was incubated in 0.25% trypsin solution (Sigma-Aldrich) at 37°C for 30 min and aspirated using a Pasteur pipette to aid cell dissociation.

Keratinocytes in the outmost stratum corneum (SC) of the epidermis are shed off and replaced by newly differentiated cells originating from epidermal stem cells located in the basal layer.

The epidermis is lifted off at the level of the dermoepidermal basement membrane.

A vertical slice was made to expose the cambium, and the exterior layer composed mostly of the epidermis was peeled off using fine forceps under a binocular microscope.

The epidermis was peeled off the dermis and cut into 5 × 5 mm pieces and fixed in acetone for 20 min at room temperature.

It seems I left my principles behind, along with a layer of epidermis that was sloughed off during a demonstration of an exfoliating mitt.