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"Syria is at the epicentre with the fastest growing problem – 12 million displaced persons, 4 million of them refugees, 2 million of them children".
It hardly even figures in Richie's crisis that he walked away from the epicentre with barely a scratch: he's more weighed down by the prospect of selling his beloved record label to some "Nazi pricks", relapsing on coke and the small business of covering up a murder.
In effect, if the directions of both approaching waves are reconstructed to come from the WSW-ESE and both epicentres of the earthquakes are placed ESE of Punta Saguerra, it is true that the localisation of the 1832 epicentre with respect to the Punta Saguerra locality is protected by the Crotone peninsula (Fig. 13) and more distant than the 1836 event.
It is the region's tobacco epicentre with over 274 million daily smokers in 2012 [ 43].
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The 12 May epicentre, together with its expected radiating pattern of shaking, is centred right at the eastern end of the 25 April rupture.
Genomic DNA isolation was performed using the MasterPure Yeast DNA Purification Kit (Epicentre Biotechnologies) with minor modifications.
Uncapped RNA was transcribed using the Ampliscribe T7 transcription kit (Epicentre Technologies) with the control linear lamda DNA provided in the kit as a template.
miRNAs were transcribed to cDNA by cDNA Synthesis Kit (Epicentre, USA) with specific reverse transcription primers (The primers were listed in Additional file 1: Table S1 ).
Genomic DNA (500 ng) was digested with 5 U EcoRI (New England Biolabs, MA) and 5 U MseI (New England Biolabs, MA) overnight at 37°C in a total volume of 45 μL containing 1× T4 DNA ligase buffer (Epicentre) supplemented with 100 μg/mL BSA.
For total mRNA-sequencing libraries, poly(A) + selected RNA was decapped with Tobacco Acid Pyrophosphatase (Epicentre) and fragmented with RNaseIII (ABI).
Among the most severe are those recorded in 1897, with the Shillong Plateau as the epicentre; in 1930, with Dhuburi as the epicentre; and in 1950, with Zayu (Rima) in Tibet at the Arunachal Pradesh border as the epicentre.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com