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The tagged cDNA constructs were purified using Qiagen MinElute columns and sample specific index sequences were added during PCR [95°C – 1 m; (95°C – 30s 55°CC – 30s 68°CC – 3 m) × 15 68°CC – 7 m; 4°C – hold] using ScriptSeq (Epicentre) index PCR primers.
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We used indexed PCR primers from the ScriptSeq Index PCR primers (Epicentre, #RSBC10948 and #SSIP1202) at a concentration of 200 nM.
We added barcode index using the Epicentre ScriptSeq Index PCR Primers.
All 60 samples were labeled using 60 different index tags (Nextera, Epicentre Biotechnologies).
On a map of the world using the Brookings index of weak states, the epicentre is self-evidently sub-Saharan Africa, particularly around Congo, with blobs of red in Iraq, Afghanistan and Myanmar.
Removal of rRNA, synthesis of cDNA and adding indices for Illumina library preperation were performed using the ScriptSeqTM Complete Kit (Bacteria) (Epicentre) following the manufacturer's protocol.
Four 6-base index sequences were used to prepare bar-coded libraries for sample multiplexing (ScriptSeq™ Index PCR Primers (Set 1); Epicentre Biotechnologies, Madison, WI).
Epicentre M13 9PC.
[Paris] : Epicentre Films [éd., distrib.], cop.
At [its] epicentre is sub-Saharan Africa".
Though nobody's quite located the epicentre.
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