Exact(2)
All MDA reactions contained 2 U/µL Repliphi polymerase, 1 x reaction buffer, 0.4 mM dNTPs, 2 mM DTT (Epicentre), 1 µM SYTO-9 (Molecular Probes) and 50 nM random hexamer primers (IDT).
Amplifications (20 μl total reaction volumes) were performed in a C1000 Thermo Cycler (Bio-Rad) using 10μL Premix B (Epicentre), 1 μl of each forward and reverse primer (10 μM), 0.5 μl of Failsafe Enzyme Mix (Epicentre), and 2 μl of DNA extraction.
Similar(58)
Epicentre M13 9PC.
RNA extraction was performed using the Epicentre MasterPure™ Complete DNA and RNA Purification Kit (Epicentre, MC85200), according to manufacturer's instructions.
(epicentre 36.6° N 89.6° W), each measuring greater than magnitude 7.0.
With a magnitude of 7.8, it was the worst to hit the country for more than 80 years, its epicentre 50 miles north-west of the capital, Kathmandu.
PCR conditions: 12 μl reaction, 1 × Buffer G (Epicentre), 50 pmol of each primer, 2.5 units Omni Klentaq polymerase (DNA Polymerase Technology) and 100 ng eDNA.
Taiwan earthquake of 1999, also called 1999 Chi Chi earthquake, earthquake that began at 1 47 am local time on Sept. 21, 1999, below an epicentre 93 miles (150 km) south of Taipei, Taiwan.
The quake was shallow, with a depth of 6.2 miles, and struck at 4.53pm local time, with the epicentre 10 miles south-west of Port-au-Prince, according to the US Geological Survey.
The quake, which was shallow, with a depth of just 6.2 miles, struck at 4.53pm local time with the epicentre 10 miles south-west of Port-au-Prince, according to the US Geological Survey.
In 2008, the Iwate-Miyagi Nairiku earthquake struck Iwate prefecture, northern Honshu, MJMA = 7.2 (epicentre 39.0283N,140.88E).
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com