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No HIF-2α (EPAS 1) was detected in normoxia.
Under hypoxic condition, the Epas 1 gene expression is enhanced.
EPAS 1 and ATF-1 were also present in extracts of hypoxic and anoxic MCF-7 cells, but our EPAS 1 and ATF-1 antibodies failed to super-shift the protein complexes bound to the wt SARE probe.
Antibodies to HIF-2α (EPAS 1), and ATF-1 did not supershift the complex bound to the SARE trimer.
A mouse monoclonal antibody to human EPAS 1 (HIF-2α) was made in-house (Wiesener et al, 1998).
Antibodies were used at a final dilution of 1 : 2000, except the EPAS 1 and β-actin antibodies which were used at 1 : 1500 and 1 : 5000.
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Photograph: Jonathan Brady / EPA 12.20pm BST Behind the scenes: journalists work in the tribune during the women's qualifier of the artistic gymnastics at the 02 North Greenwich Arena.
Pathogen contamination clearly dominates the causes of impairment (U.S. EPA [2014a], [2014b], [2014c]).
Specific quality requirements defined by EPA 314.0 will be discussed later.
In hypoxic water, dissolved oxygen levels can be less than 2 – 3 ppm (U.S. EPA [2014c]).
EPA 314.0 defines the PGF as an indicator for the analytical column's hydrophobicity.
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