When they applied the enzyme solution to bloodstained white denim, they found that 15 to 20percentt more blood was removed than by plain washing.
Ansite, J. et al. Purified human pancreatic islets, CIT enzyme solution roche enzymes — standard operating procedure of the NIH Clinical Islet Transplantation Consortium.
Ansite, J. et al. Purified human pancreatic islets, CIT enzyme solution vitacyte enzymes and vitacyte/serva enzymes combination — standard operating procedure of the NIH Clinical Islet Transplantation Consortium.
Isolated tissues were washed three times and incubated at 37 °C in a dissociation enzyme solution with occasinal pipetting to a single-cell suspension.
Ansite, J. et al. Purified human pancreatic islets, CIT enzyme solution: serva enzymes proportional units collagenase & neutral protease — standard operating procedure of the NIH Clinical Islet Transplantation Consortium.
The enzyme solution was supplemented with 1 mg/ml collagenase A (Roche Applied Science, Meylan, France) and 5 mg/ml bovine serum albumin BSA (Sigma, Lyon, France).
A negative control reaction (non-enzymatic background) was set up in the same conditions, with buffer instead of the enzyme solution.
However, only about 5.4 wt% and 12.9 wt% of silk samples after space exposure dissolved in the LiBr solution and the XIV-protease enzyme solution, respectively.
For labeling, sections were incubated in TUNEL enzyme solution for 1 h at 37 °C in the dark and counterstained with DAPI.
After dialysis, the enzyme solution was lyophilized.
Controls were assayed without enzyme solution.
