He did not try to develop experimental instruments to attack the gene-enzyme relations themselves implicated in the process.
These relations describe enzyme-enzyme relations, protein-protein interactions, interactions of transcription factors and genes, protein-compound interactions and links to other pathways.
First, because the study by Plata et al. aimed at predicting the essentiality of genes, whereas the other studies assessed the essentiality of enzymes, the predictions made do not overlap when gene-to-enzyme relations are not one-to-one.
When expressing cellulases in plants, it is important to consider all factors concerning the expression levels and relative activity of the enzyme in relation to its localization within the plant.
The 17βHSD2 immunoreactivity was also examined in order to further explore the significance of this particular enzyme with relation to androgen actions.
This resulted from the fact that the PTE addition to a diet with FOS significantly reduced the activity of this enzyme in relation to the diet with cellulose, whilst PTW addition did not evoke such an effect.
This provided the basis for studying this enzyme in relation to lung cancer risk, and some studies indeed described an increased risk in individuals lacking this enzyme, but no such effects could be found in a sufficiently powerful meta-analysis [ 87].
Dobrinski et al. [17] examined four CA enzymes (α, β, γ and CsoSCA) from the deep sea proteobacterium Thiomicrospira crunogena and suggested different roles for each of the enzymes in relation to carbon fixation capabilities and survival mechanisms of the microorganism.
However, because less UV-resistant Bacillus species also have photolyase enzymes, the relation of photolyase to enhanced UV resistance is not clear.
Few studies have addressed the gene-gene interaction between phase I and II enzymes in relation to colorectal carcinogenesis [ 20, 21, 34].
