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A central composite design pattern was used to understand the relationship between several variables (pH, temperature, reaction time, and enzyme quantity) and two responses (removal of ferulic acid derivatives and COD decrease).
The results showed that the optimum conditions were an enzyme quantity of 0.5 g (the mass ratio of cellulase and pectinase was 1 2, and the enzyme activity was 60 U/mg), enzymolysis pH of 4.5, and temperature of ultrasound of 45 °C.
Enzyme activity is defined as the enzyme quantity needed to degrade 1 μmol of substrate per minute.
One unit of 2,5-DKG reductase activity is defined as the enzyme quantity required to reduce 1 μmol of 2,5-DKG per min under assay conditions, which is equivalent to the production of 1 μmol of NADP+ per min (Kaswurm et al. 2012 ).
Immunoblotting was used to compare SodA enzyme quantity.
In E. coli, the flux between the TCA and glyoxylate cycles is controlled by modification of isocitrate dehydrogenase rather than on enzyme quantity [23].
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For biosensor design, the DDI method allows the use of small enzyme quantities for monitoring the reaction while allowing both enzyme and surface to be regenerated for subsequent usage.
Gene expression involving changes in enzyme quantities cannot explain the observed fast (about 10-min) responses to changes in ci.
In addition, changes in substrate/product concentrations or metabolic regulations can influence the reaction fluxes irrespective to the enzyme quantities.
Not accounting for enzyme quantities but only reaction fluxes imposes a second limitation to genome-scale models.
Enzyme productivity (quantity of sugar produced per quantity of enzyme applied) is one of the best methods for comparison of enzyme efficiency.
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