Sentence examples for enzyme quantification from inspiring English sources

Samples were homogenized in 30 volumes (w/v) of ice-cold mannitol (50 mM), Tris HCl buffer (2 mM) pH 7.0, at a maximum speed for 30 s (IKA, Ultra-turrax®, USA); then, 100 μl of 0.1 M CaCl2 was added to the homogenate, which was centrifuged at 10,000×g for 10 min at 4 °C and the supernatants collected and stored in aliquots at − 80 °C for further enzyme quantification (Gisbert et al. 2009).

Details of enzyme quantification can be found in the Supporting Information.

If cirrhotic changes already occurred, liver enzyme quantifications every 3 months with ultrasound of the abdomen and Alpha-Fatal Protein measurements twice a year are performed at our institution.

Results from the study of growth, enzyme activities, quantification of breakdown products from the enzymes, and the nature of the remaining undigested sorghum should enhance our understanding of the plant cell wall degradation process and help us to devise ways to accelerate the process of lignocellulosic bioconversion using in vitro enzyme mixtures.

The cell pellets were used for the enzyme assays and quantification of transcriptional level as described below.

The copy number of clade II was determined using polymerase chain reaction (PCR) amplification followed by restriction enzyme digestion and quantification of the two digested products.

These methods include but are not limited to enzyme activity assays, quantification of protein protein interactions (Selbach and Mann, 2006), or additional RPPA readouts under specific perturbation conditions.

This is followed by restriction enzyme digestion and quantification of the two peaks produces a ratio of cut to uncut products.

Furthermore, secreted hiAP can be used as an instrument for scientific purposes as well as a reporter enzyme allowing the quantification of measures that are planned to optimize the ciliate expression system.

Additionally, the fact that ciliates do not secrete an endogenous alkaline phosphatase provides the possibility to use the truncated hiAP as a reporter enzyme, allowing the quantification of measures that will be necessary for further optimization of the host strains and the fermentation processes.

The assay facilitates mechanistic studies of triphosphohydrolase enzymes and the quantification of off-target effects of nucleotide-based antiviral and chemotherapeutic agents.