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The procedure for measuring SSS activity used enzyme preparation 2 rather than enzyme preparation 1.
The supernatants were used for crude enzyme preparation.
The final enzyme preparation was kept in the same buffer (1 ml) at 4°C.
The enzyme preparation was homogeneous as judged by sodium dodecyl polyacrylamide gel electrophoresis (SDS-PAGE).
The supernatant, referred to as enzyme preparation two, was used to determine GBSS activity.
The purity of the purified enzyme preparation was checked by SDS-PAGE.
SEM analysis demonstrated significant morphological changes in the enzyme preparation as a result of ultrasonication.
The cell-free supernatant was collected as crude enzyme preparation and subjected to purification procedures.
Permeabilized microbial cells can be used as a crude enzyme preparation for industrial applications.
Yet it gives the enzyme preparation suitable for most screening purposes.
The specific activity of the obtained solid enzyme preparation is comparable to a commercial preparation.
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