The highly abundant seed transcripts encode a variety of well-characterized proteins such as seed storage proteins, protease inhibitors, proteases, acyl lipid enzymes, oil body proteins and late embryogenesis abundant (LEA) proteins, and as well proteins of unknown functions.
The optimisation results predicted that optimum reaction conditions for preparing enzyme interesterified oil, having minimum kinematic viscosity (2.63 × 10−6 m2 s−1) and maximum heat transfer coefficient (262.0 °C−1 °C−1) were at 62% RBO, temperature 65 °C, enzyme concentration 10% (w/w) and time 6.4 h.
The results suggest the possibility of applying an in situ EOR approach using a combination of Enzyme Enhanced Oil Recovery (EEOR) and Microbial Enhanced Oil Recovery MEORR) methods, with this lipase-esterase producing Bacillus licheniformis isolate, which is indigenous to one of the wells in this oil field.
Therefore, we predict that DGAT is a crucial enzyme for oil production of soybean a suggestion also supported by previous study [ 7].
Additionally, the emerging contributions of genetically-engineered microorganisms for MEOR purposes (GEMEOR) and enzyme-enhanced oil recovery (EEOR) are also analyzed.
In this study, response surface methodology was applied to optimize process variables like temperature, pH, enzyme concentration (mg/g oil), and buffer concentration (g/g oil) for hydrolysis of castor oil using Candida rugosa lipase.
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The optimal conditions for transesterification obtained were: substrate molar ratio of 7.5 1 ethanol:soybean oil; enzyme content of 25% (weight of oil); and 80% of TLL in the mixture of biocatalysts.
For hydrolysis, the optimal conditions were: substrate molar ratio of 3 1 water:soybean oil; enzyme content of 25% (weight of oil); and 65% of TLL in the mixture of biocatalysts.
