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The control was enzyme mixed with distilled water instead of inhibitors.
A 2-fold increase in ethanol titre (23 g·L−1) (Table 3) was obtained in lab scale bioreactor on scaling up the shake flask SSF (11.2 g·L−1) (Table 3) with mixed enzyme mixed culture system using 5% (w/v) wild grass.
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The experiment was performed in three groups respectively using extra-enzyme, intra-enzyme and extra-enzyme mixed with intra-enzyme at the ratio of 1 1.
For each substrate, extra-enzyme mixed with intra-enzyme contributed higher levels of xylose releasing than those by extra-enzyme and intra-enzyme respective hydrolysis.
At first the discharge's main function was to lubricate the snake's food, but with the passage of time, some enzymes mixed with secretions allowing for the emergence of more elaborate and potentially toxic proteins, used in the immobilization of prey.
Although we could not distinguish ChiA and ChiB visualized with HS-AFM, the movement of opposite direction appeared only after the two kinds of enzyme were mixed, as shown in Supplementary Movie 4.
The purified peptidase M15A enzyme was mixed with 2× sample-refolding buffer (0.5 mM Tris HCl pH 6.8, 20 % glycerol, 0.2 % bromophenol blue), then loaded into the SDS-PAGE containing E. coli.
In the first moment after an enzyme is mixed with substrate, no product has been formed and no intermediates exist.
A dilute solution with or without labeled enzyme was mixed with MBM and this suspension was quickly transferred to a viewing dish.
The purified BGL-CBD enzyme was mixed with microcrystalline cellulose in the quantitative experiments.
A control was also included where the enzyme was mixed with distilled water instead of metal solution.
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