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Shaken culture was washed with PBS twice and then NADH enzyme levels were detected using a Enzychrom kit (Bioassay Systems, CA, USA).
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Gene expression levels were detected using RT-PCR analysis.
(d) Cellular superoxide levels were detected using HEt.
Cytokine levels were detected using ELISA.
No thrombocytopenia or elevated liver enzyme levels were detected.
Chitinolytic and chitosanolytic enzyme activities were detected using a dot activity assay as described previously [ 26].
The enzymes were detected using 12%% polyacrylamide SDS-PAGE, stained with Coomassie brilliant G-250.
Redox enzymes in cryostat sections were detected using the Nadi reaction and according to Nachlas.
The rest were detected using enzymes that cut one or other allelic copy.
The T. vaginalis amplicons were detected using an enzyme immuno assay (EIA).
The CYP2D6 and SULT1A1 polymorphisms were detected using restriction enzymes.
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