Sentence examples for enzyme in each sample was from inspiring English sources
The amount of the enzyme in each sample was calculated according to a positive control loaded on the same gel, active elastase (Sigma-Aldrich-cat. number E-8140)/acathepsinhepsin G (Fitzgerald Industries International, USA).
The amount of enzyme present in each sample was determined by western blotting and densitometry analysis by the use of ImageLab v3.0 with antibodies against hNaa10 and hNaa15.
The absorbance of each sample was measured in an enzyme-linked immunosorbent assay reader at 370 nm.
Because the refolding efficiencies of mutant recombinant proteins differed from sample to sample, the amount of active enzyme in the sample was estimated by CA. To compare the peptide-cleaving efficiency of the mutant recombinant proteins, the activities were normalized by CA, and expressed as nmol min-1 CA-1.
The abundance of each gene for an aromatic compound-degrading enzyme in a metagenomic sample was normalized by (i) the amino acid length of the enzyme and (ii) the average abundance of the gyrB-added USCGs.
Ethidium bromide-stained bands were quantified with a VersaDoc 5000 and Quantity One software (Bio-Rad, Hercules, CA, USA) and percentage of methylated restriction enzyme sites in each genomic sample was calculated from the ratio between the enzyme-cleaved PCR product and the total amount of PCR product.
Although an extreme abnormal activation of this enzyme in breast carcinoma samples was described already 35 years ago [ 58], ACLY contribution to breast cancer development and progression is yet unclear clear and requires further investigation.
Quantification of enzyme activity in samples was determined using NIH imaging software [ 25].
The activity of the CLEAs declined to ~60% of the initial activity over the five steps, but the heat activation detected in the free enzyme sample was also observed in the CLEA preparation (Fig. 5).
Dehydrogenase enzyme activity in soil samples was determined by following the procedures as described by Casida et al. (1964).
If the enzyme activity in the samples was not fully stopped, MTX in the sample might continue to be degraded after collection.
