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Seven inositol stereoisomers were tested, all of which were found to be substrates of the enzyme, generating the corresponding phosphatidylinositols.
The newly formed dsDNAs are then recognized and cleaved by the nicking enzyme, generating new DNA triggers with the same sequence as the target miRNA, which hybridize with intact HPs to initiate new extension reactions.
Analog 4A was found to irreversibly inactivate the enzyme generating competitive- and time-dependent inhibition of activity accompanied by a Ki value of 2 μM and kinact of 0.3 min−1.
The secretion leads, by two pathways, depending on the substance encountered, to the cleavage and activation of the proclotting enzyme generating the clotting enzyme.
The direction was defined as starting from the enzyme generating the metabolite to the enzyme consuming it.
Phospholipase D has been identified as an enzyme generating secondary messengers in plants, triggering defense against bacterial attacks [ 21].
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The enzyme generated a fungicidal activity against Saccharomyces cerevisiae in a time- and a dose-dependent fashion using alliin as a stable precursor.
We have analyzed PC samples from Staphylococcus aureus while the enzyme generates oxaloacetate, expecting PC tetramers to display the conformational landscape relevant for its functioning.
Figure 2 shows the MAIDI-TOF MS spectrum of the enzyme generated from the in-gel trypsin digestion, and Additional file 1: Table S1 lists the obtained PMF peaks.
LDH release was expressed as a percentage of the total amount of the enzyme generated through complete cellular lysis using Triton X-100 (9% [v/v]).
The SHMT1 enzyme generates methyleneTHF for thymidylate and methionine biosynthesis, but isotope tracer studies indicate that SHMT1 preferentially partitions methyleneTHF to thymidylate biosynthesis [6].
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