Sentence examples for enzyme fingerprinting from inspiring English sources

The current trend is to assemble many such assays in parallel for enzyme profiling and enzyme fingerprinting.

In traditional hierarchical sequencing we would then construct a high-coverage clone library from these fragments and select a subset of the clones to form a tiling path with minimal overlap using techniques such as restriction enzyme fingerprinting.

Enzyme fingerprinting results identified acetyl xylan esterases as key to the enhanced ruminal digestion.

Twelve intragenic SNPs were determined by a method based on fluorescent restriction enzyme fingerprinting single-strand conformation polymorphism (F-REF-SSCP).

In the article by A. Badhan et al., they have utilized enzyme fingerprinting to identify cell wall components resistant to total tract digestion.

Based on the results from the enzyme fingerprinting (experiment 1) we selected recombinant enzymes (namely acetyl xylan esterase AXE16A_ASPNG and AXE16B_ASPNG, polygalacturonase PGA28A_ASPNG, arabinofuranosidase ABF54B_ASPNG, and ferulic acid esterase FAE 1a) and endoglucanase EGL7A_THITE for prehydrolysis with an aim to increase the sugar yield from substrates exposed to mixed rumen enzymes.

Such contigs are similar to the whole-genome sequencing contigs, but they are constructed using overlapping restriction enzyme fingerprints rather than overlapping sequences themselves for the whole-genome sequence assemblies.

Pools of overgo probes were then hybridized to the white-throated sparrow library, and probe content and restriction enzyme fingerprint maps of each targeted region were constructed using previously described methods (Thomas et al. 2002).

The current genomic resources include: a BAC library [ 4], restriction enzyme fingerprint physical map comprising 223,781 BACs in ~4,300 contigs [ 5], 207,869 BAC-end sequences that cover ~3.5% of the genome sequence, a linkage map with ~1,600 markers, ~600 of which are integrated with the physical map [ 6], and > 432,000 ESTs [ 7, 8].

We chose a two-tiered PCR approach, first using a restriction enzyme digest fingerprinting strategy followed by multiplex PCR for serotypes classified as NT by the RFLP methods [ 9, 10].

Based on probe-content and restriction-enzyme fingerprint BAC contigs at the targeted loci we observed an average clone depth of 10.2×, which is very close to the depth of coverage predicted for the library.