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CocH expressed by adenoviral gene transfer vector enables durable and effective levels of enzyme expression in liver and plasma.
Nilsson, R. et al. Metabolic enzyme expression highlights a key role for MTHFD2 and the mitochondrial folate pathway in cancer.
This work demonstrates the utility of combining systems and synthetic biology to analyze and optimize heterologous enzyme expression.
The optimization of engineered metabolic pathways requires careful control over the levels and timing of metabolic enzyme expression.
Podrabsky, J.E., Javillonar, C., Hand Steven, C. & Crawford, D.L. Intraspecific variation in aerobic metabolism and glycolytic enzyme expression in heart ventricles.
For both enzymes, silent mRNA mutations were identified that increased enzyme expression.
During acute infection, the distribution of viral replication differed from the distribution of GUSB enzyme expression.
Enzyme expression varied inversely with dietary changes according to the developmental pattern.
Recent approaches to these engineering objectives have made use of molecular tools to advance de novo pathway identification, tunable enzyme expression, and rapid pathway construction.
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Previously, we developed 6- tri-fluoroacetamido -1-hexanoicanilide ([18F]TFAHA), a highly-selective radiotracer for quantitative imaging of HDAC class IIa enzyme expression-activity in vivo using PET/CT/(MRI)42.
The cellulolytic enzyme expression-optimized strain MT8-1/cocδBEC3 exhibithe thighestethanolanol productivity.
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