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Five samples were taken of each formula for enzyme determination upon completion of substrate colonization.
Results of further investigations using spinal magnetic resonance imaging, intravenous pyelography, renal CT, cardiac enzyme determination were within their normal ranges.
The designed probes demonstrated long-term photostability and very good sensitivity in a chymotrypsin assay, allowing quantitative enzyme determination with the advantage of ratiometric measurement.
Tissue homogenates were incubated for 60 min at 0°C for complete enzyme extraction and centrifuged at 13,000×g for 20 min. The supernatant was used for enzyme determination.
It was the purified protease identified via enzyme determination and MS/MS.
Crude extracts were filtered over Sephadex G-25 (NAmershamrsham Biosciences, Uppsala, Sweden) before enzyme determination.
Similar(52)
Such quantitative correlations could not be made in the spleen as representative matched samples were not available, since GC and enzyme determinations consumed nearly the entire spleen.
The supernatant fraction was used for antioxidant enzyme determinations.
These lesions were detected only because the patients were on MTX with monthly liver enzyme determinations.
All enzyme determinations were performed in triplicate in at least three independent experiments using a NovoStar MBG Labtech microplate instrument.
No convincing results have yet been presented that would favour the use of enzyme determinations in assessing pre- and post-thaw semen quality.
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