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All together, optimization of each enzyme component is attractive for lowering enzyme loadings; preparing such type of complex mixtures is quite challenging.
The effects of enzymatic treatments on pulp and paper properties showed that the type of enzyme component is probably very important.
Enzymatic saccharification of the pre-treated lignocellulosic biomass depends on the hydrolytic efficiency of each individual cellulase enzyme component and their synergistic action as a multi-cellulase complex for breakdown of celluloses and hemicelluloses (Van Dyk and Pletchke 2012).
In addition, the new Curriculum for Excellence Advanced Higher Biology has content related to protein structure (covered, to an extent, by the "ENZYME" component of 4273π) and scientific investigative approaches (Scottish Qualifications Authority 2015b).
Saccharification of the pretreated lignocellulosic biomass depends mainly on the hydrolytic efficiency of each individual cellulase enzyme component present in the multi-cellulase complex and their synergistic action towards the breakdown of insoluble celluloses and hemicelluloses into simple reducing sugars [6].
The most efficient single enzyme component here was T. reesei EG TrCel7B.
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How efficient, however, are the individual enzyme components?
Mixture design showed positive interactions among the enzyme components.
Moreover, adding requisite amounts of enzyme components that were deficient in the cocktail mixture may further improve the overall process efficiency.
Thus, it was concluded that crude cellulose-degrading enzymes produced by the A. terreus D34 with mixed growth substrates produced efficient crude enzyme mixture with the required enzyme components in appropriate proportions to yield high reducing sugars.
These studies provide a clear evidence of the production of multiple cellulase proteins and their correlation with the corresponding enzyme activities with a complex synergetic mechanism for combined action between the enzyme components for effective lignocellulosic breakdown.
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