The ethyl ferulate/soybean oil substrate was fed through the four, packed-bed, enzyme columns in series with each column fitted with a recirculation pump, which improved conversion and throughput compared to smaller, batch bioreactor systems.
Profiles numbered from a to k were listed in Figure 2. The "No. of Enzymes" column means how many enzymes of each pathway are in the array, the "No. of Enzymes submitted" column means how many enzymes belong to each profile.
Figure 1, in the Hyal-like enzymes column, illustrates the evolution of the Hyal-like sequences: From one in C. elegans, to three in the sea squirt, to six such sequences in the zebra fish.
If the set of the releasing enzymes is identical for all marking enzymes, one column can be used for all digestions.
However, an identical trend is also seen for the number of enzymes (right column of Figure 5).
Furthermore, to obtain a high methyl ester content above 96% continuously, long-term lipase stability was confirmed by operating a bench-scale PBR system for 550 h, in which the intermediates containing methyl esters and residual glycerides were fed into the enzyme-packed columns connected in series.
The activity and abundance datasets were each organized into a matrix, with rows corresponding to enzymes and columns corresponding to studies.
T-RFLP profiles of bacterial 16 S rRNA amplicons digested with the restriction enzyme Msp I (column A).
Plasmids were linearized by treatment with the corresponding restriction enzyme followed by column purification (Roche).
The method we used does not involve any fixation of the target enzyme onto a column, so that it preserves its biochemical integrity.
