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In this enzyme-centric perspective, we discuss the scientific and technological challenges that could accelerate the adoption of combinatorial biosynthesis as a method of choice for the preparation of encoded libraries of bioactive small molecules.
The mathematical model of RNA splicing was built using the Enzyme-Centric approach.
We also examined the impacts of enzyme knockouts from the enzyme-centric view with our method.
The distances of effects could be directly counted from the enzyme-centric network.
Modularization of such enzyme-centric graph categorizes enzymes into different functional groups.
Simulations using the integrated, non-linear Enzyme-Centric model uncover the purposes of these designs.
We could explicitly see the interactions among enzymes from the enzyme-centric view (Additional file 1).
This result suggests that enzyme-centric features make more contributions to our proposed interactions network of substrate-enzyme-product.
It is important to demonstrate the difference between the non-linear, enzyme-centric model and linear conversion model.
In contrast, with the enzyme-centric model (Fig 3D), products (isoleucine and threonine) are decreased as substrate (Pyr) increases.
Second, alternatively one can focus on the catalytic functions of enzymes and construct an "enzyme-centric" network.
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