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Enzyme blends were identified, outperforming the individual commercial preparations.
In Exp. 1, effects of enzyme blends (amylase, protease and xylanase) at different dose rates [high (H) vs. medium (M) vs. low (L)] on the coefficient of apparent digestibility (CAD) were investigated in vitro using a two-stage enzyme incubation method.
This developing practice is based on the understanding that the effective degradation of the complex structure of lignocellulose requires not only cellulases, but also supplementary enzyme blends of ligninases (laccases), hemicellulases, and accessory proteins, depending on the morphological characteristics of the lignocellulosic biomass.
Enzyme blends and optimization are required to speed up enzymatic hydrolysis to make the process commercially viable.
Synergy among enzymes from individual enzyme extracts is also another advantage to utilization of these enzyme blends [ 7, 8].
The use of enzyme blends showed the synergistic effect of the enzymes produced by these two fungi.
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Linetsky, E. et al. Human islet isolation using a new enzyme blend.
For an efficient hydrolysis of lignocellulosic materials a different ratio might be desirable and this may be achieved just by adding further copies of either gene in the fusion construct in order to obtain an optimized enzyme blend.
FastStart High Fidelity Enzyme Blend (Roche Diagnostics, Indianapolis, IN) was used in both rounds of PCR.
Enzyme blend mixing of fraction C15 and C20 gave higher activity than testing the two fractions individually.
aXylose and arabinose at a low concentration (0.03 g/L; 0.12% yield) were released solely for the hydrolysis performed by the A. cellulolyticus- A. awamori enzyme blend.
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