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J.K. and H.J.H. designed the enzyme assay and kinetics studies.
The glucose content measurement was performed in 96-well plates using a coupled enzyme assay method.
The primary surface detection method used was an enzyme assay with alkaline phosphatase labelled streptavidin (Fig. 3a).
The substrate for the CASP3 enzyme assay was purchased from Promega (Caspase-Glo 3/7 Assay System, Promega, Madison, WI).
Voznyi Ya, V. et al. Applications of a new fluorimetric enzyme assay for the diagnosis of aspartylglucosaminuria.
We purified Azi_1.1 and Ery_1, and verified azithromycin and telithromycin phosphorylation in an in vitro enzyme assay (Supplementary Fig. 5).
Alkaline phosphates enzyme assay also reveals that the presence of graphene oxide nanosheets moderately hampers osteogenesis of the cultured cells.
(a) Inkjet printing of three different FSL-biotin constructs (Fig. 1) on three different materials; paper (left), polyester (middle) and 316 stainless steel (right), visualised by enzyme assay.
H. sapiens DHFR (HsDHFR) and P. vivax DHFR (PvDHFR) were thawed and stored at 4 °C for use in the enzyme assay.
In this study, however, we were unable to conduct UGT1A1 enzyme assay with bilirubin due to its lack of stability against light.
Optimization of this nanochannel sensor could lead to a quick-response, highly sensitive, and label-free sensor for enzyme assay and kinetic studies.
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