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Importance of the interface between these domains and particularly of the 5 salt bridges for enzyme activity was established for protozoan OpdBs.
Statistical significance between genotypes for mRNA levels, quantification of mineralization, and quantification of AP enzyme activity was established by use of the Student's t-test.
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This concept of setting grades for elevations of serum enzyme activities was established in 1983 when the National Cancer Institute (NCI) of the United States National Institutes of Health (NIH) first published their Common Toxicity Criteria (CTC) for adverse events.
In the optimization studies of the biosensor the most suitable enzyme activity was found as 2.5 U/cm2 for pyruvate oxidase, and also phosphate buffer (pH 7.0; 50 mM) and 35 °C were established as providing the optimum working conditions.
The topoisomerase activity was established when the compound was added together with DNA and enzyme leading to an observed inhibition of relaxation activity at various concentrations.
A non-radioactive CPT1 enzyme activity assay was established and siRNA transfections were performed to transiently knock down specific targets in the AMPK pathway.
The analytical performances of the enzyme substrate determination in a wide concentration range (0.1 20 μmol dm−3 of acetylcholine) and different ACh activities were established.
The enzyme activity was calculated using both a previously established standard curve with d-glucose (Carl Roth, Karlsruhe, Germany) for the determination of the amylase and cellulose activity and a previously established standard curve with d-xylose (Adlersdorf, Berlin, Germany) for the determination of the xylanase activity in a concentration range of 3 to 20 μmol/mL.
Preliminary determinations established that enzyme activity was linear with time and tissue concentration under these conditions.
Enzyme activity was assayed using 2,2′-azino-bis 3-ethylthiazoline-6-sulfonate 2,2′-azino-bis 3-ethylthiazoline-6-sulfonate 2,2′-azino-bis 3-ethylthiazoline-6-sulfonate 2,2′-azino-bis 3-ethylthiazoline-6-sulfonate 2,2′-azino-bis 3-ethylthiazoline-6-sulfonate 2,2′-azino-bis 3-ethylthiazoline-6-sulfonate
The enzyme activity was measured in the presence of NADH at the indicated concentrations.
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