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After test-cleaning in a few marginal areas, Duffy worked his way inch by inch across the picture, cleaning off the accumulated dirt and grime with cotton swabs dipped in a "mild enzymatic solution," otherwise known as spit.
The hearts were retrogradely perfused through the aorta, first with a Krebs-Henseleit (KH) solution supplemented with 10 mmol/L taurine for 5 minutes, then with enzymatic solution for 20 minutes.
An enzymatic solution containing d-lactate dehydrogenase, diaphorase and NAD-dextran was further confined on the sensing electrode using a semi-permeable membrane.
The results obtained showed high removal degrees (up to 70%, after four hours), even when limited amounts of enzymatic solution were used.
This enzymatic solution is often used to reproduce in laboratory the ennoblement occuring in non-sterile aerated aqueous environments because of the biofilm settlement on the surface of the metallic material.
This article deals with the interaction between the passive layer formed on UNS S30403 and S31254 stainless steels and an enzymatic solution containing glucose oxidase (GOx) and its substrate d-glucose.
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We obtained the D-periosteum by employing a combination of commonly used decellularization processes, which include physical methods as well as chemical and enzymatic solutions.
Cell culture media and reagents, enzymatic solutions and antibiotics were from Gibco/Life Technologies (Saint Aubin, France).
Having colonized virtually every environment, bacteria and archaea have evolved enzymatic solutions for a wide range of metabolic biochemical transformations [ 1, 2].
A great diversity in the metabolic and enzymatic solutions used to produce this molecule is observed, and the picture may be even more complex as some genes are still missing in specific lineages.
For flow cytometry analysis of cell surface molecules, cells were detached using a non-enzymatic solution (Invitrogen), washed and stained with saturating concentrations of primary mAb (10 µg/ml).
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