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Sato, H. Enzymatic procedure for site-specific PEGylation of proteins.
A simple enzymatic procedure is used to modify the molecular architecture of a lightly chemically crosslinked galactomannan hydrogel as well as a model drug galactomannan oligomer conjugate, fluoroisocynate (FITC) tagged guar oligomer, to entrap the model drug.
In brief, FDP concentration was measured by an enzymatic procedure of Racker (Bergmeyer 2012) using a commercial JSAY139 FDP kit (Jie Shi Kang Biotech Co., Ltd. China).
Serum total cholesterol was determined by an automated enzymatic procedure using the Roche CHOD-PAP reagent agent, and HDL was measured with the Roche HDL cholesterol assay using PEG-modified enzymes and dextran sulfate.
Plasma glucose and β-OHB concentrations were measured spectrophotometrically using the StanBio® Enzymatic Glucose Assay (1075-102) (StanBio Laboratory, Boerne, TX, USA) and a modification of the Williamson et al., enzymatic procedure [57], respectively.
Plasma lipids were measured at CTQ using standard enzymatic procedure.
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Thus, chemical and enzymatic procedures were developed for the radiolabeling of highly purified glycosphingolipids.
The platform combines miniaturized, two-dimensional chip arrays with proven high-fidelity enzymatic procedures and matrix-assisted laser-desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry.
The use of biological catalysts for industrial scale synthetic chemistry is highly attractive, given their cost effectiveness, high specificity that obviates the need for protecting group chemistry, and the environmentally benign nature of enzymatic procedures.
Total cholesterol and triglyceride concentrations were determined by enzymatic procedures (Hitachi 747 chemistry analyzer, Hitachi, Tokyo, Japan).
Serum creatinine and lipid profiles, including total cholesterol (TC), TG, HDL-c and low density lipoprotein cholesterol (LDL-c) were determined by enzymatic procedures using an autoanalyser (Hitachi 7600 020; Hitachi, Tokyo, Japan).
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