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Fluorescent microscope (Nikon) was used with Envision system.
Immunoreactions were revealed with the monoclonal EnVision system (Dako, Denmark) using 3-3'-diamonobenzidine as chromogen.
Secondary Ab: Dako EnVision System labeled Polymer-HRP anti rabbit (DAKO, # K4003).
Immunohistochemical staining of tissue sections was performed using the Envision System HRP mouse (Dako, Glostrup, Denmark).
Antibody binding was visualized by the Dako Envision system (cat #K4011).
Immunohistochemistry staining was performed using the EnVision™ system (EnVision system, Dako, USA) [ 22].
Similar(32)
Staining was achieved with DakoCytomation EnVision System-HRP (DAB) kit.
For secondary polymer, Envision system-HRP was used.
Next, the slides were incubated with substrates for Envision system-HRP until desired staining intensity developed.
Secondary antibodies were Envision+ System- HRP Labeled Polymer Anti-Rabbit, and Envision+ System- HRP Labeled Polymer Anti-Mouse (Dako North America, Inc., Carpinteria, CA, USA).
Luciferase activity was measured using the PerkinElmer EnVision™ system.
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