As environmental specimens we used an agricultural sandy soil (arable sandy loam, ASL), and white quartz sand (Sigma-Aldrich).
As shown in figure 1A (codes L1 to L4), of the 29 environmental specimens that were investigated for P. aeruginosa contamination, only 4 yielded visible growth on selective Pseudosel agar plates.
Sabouraud dextrose agar (SDA), Mycosel ® agar, SDA fortified with an enhanced panel of antibacterials (amikacin, ampicillin, carbenicillin, cephelixin, cefazolin, colistin methanesulfonate, kanamycin, ofloxacin, streptomycin, tetracycline, and vancomycin), and Rose Bengal agar were used for the isolation and purification of fungi from the bat tissues and environmental specimens.
Milk specimens as well as environmental specimens were collected.
Case-related and environmental specimens were collected for etiologic detection.
Cultures of all environmental specimens were negative for B. anthracis.
Two sample types, clinical and environmental specimens, were used for detection of NV GI and GII.
Environmental specimens were collected from groundwater in the Republic of Korea during June and October 2013.
Biologic and environmental specimens should be carefully collected, processed, and banked in multiple aliquots.
On the hospital side, environmental specimens were taken after thorough clean-up.
Of 314 external environmental specimens, the overall positive detection rate of H7N9 nucleic acid was 28.98%.
