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For environmental sequence types, numbers in parenthesis indicate the accession numbers given in GenBank.
For environmental sequence types, numbers in < and > symbols indicate the total number of sequences in the corresponding library from which they were retrieved.
To assess whether primers used to amplify 18S rDNA might be used to fingerprint genetic diversity in nematode communities in soil, the environmental sequence data were used to design a second set of primers carrying a GC-clamp.
Cephaloidophora cf. communis (both sequences) and its closely related environmental sequence were 12.4% dissimilar; Thiriotia pugettiae and its closely related environmental sequence was 15.6% dissimilar.
Also similar to the rock phylotype was an environmental sequence from hydrothermal vent fluids [21].
Identification of every environmental sequence to species level, however, may not always be necessary for many ecological and evolutionary questions.
Genome-level phylogenetic analysis was accomplished using fragment recruitment of environmental sequence reads to reference microbial genomes [5].
Homologues of AbSV proteins in the environmental sequence data were detected by searching the NCBI environmental data set using BLASTP.
Sequence divergence between the two H. cf. longissima sequences and the environmental sequence Sey010 from freshwater sediment of a river in Switzerland was 0.41%.
For example, environmental sequence data from the lowest diversity site (MHS) provided ∼4x coverage relative to the Sulfurihydrogenibium sp. Y03AOP1 or S. yellowstonensis genomes.
The environmental sequence (FJ803977) differed at two and four nucleotide sites from the two known R. vinosa sequences, AY061724 and AF418638, respectively.
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