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Do divergent lineages of environmental genes point to divergent, deep-branching lineages of organismal hosts?
Metagenomics analysis comprises environmental single-gene surveys and random shotgun studies of all environmental genes.
In our networks, most divergent environmental genes grouped exclusively with uncultured relatives, in maximal cliques.
Interestingly, we observed such divergent lineages of environmental genes in human microbial gut samples.
This could be due to the fact that duplication or deletion of nonenvironmental genes might be more frequently deleterious than copy number changes of environmental genes [ 88].
The difference between the genetic diversity of these environmental genes and that of cultured microorganisms, for these 82 important markers, raises a general problem.
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Working with local shamans, plant collectors, scientists, and others, they examine seeds, nuts, berries and bark for potent medicines in an environmental "gene rush".
A wide variety of environmental gene tags (EGTs) from several metabolic routes were found.
These include the development of environmental gene tags and the serial analysis of 16S rRNA gene sequence tags.
The dataset generated by pyrosequencing of a fosmid library was mined to identify environmental gene tags (EGTs) assigned to FeFe-hydrogenase.
Another method that is being increasingly employed in environmental gene expression studies is SMART PCR, which generates and amplifies double-stranded (ds) complementary DNA (cDNA) from sscDNA using PCR, prior to gene-specific PCR.
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