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Seed devitalisation was measured by germinating seeds in soil bins in a controlled environment room.
The laboratories at Rothera are very well equipped with, for example, analytical chemistry and microbiology labs, a controlled environment room, an aquarium, and a modern diving and boating facility.
The absence of proximal cues forced the participants to rely on distal features of the environment (room walls, landscape elements) and tested their ability to use allocentric (world-based) navigational strategies requiring cognitive mapping.
(a) AgNW suspension drop down on Si substrate and spin-coating at 8,000 rpm for 30 s; (b) dry in ambient environment room temperature; (c) NOA63 drop down and spin-coating for first spinning at 300 rpm for 15 s, second spinning at 800 rpm for 15 s; (d) after spin-coating; (e) UV curing at 300 W (370 nm) for 4 min; (f) peel-off process.
Each individual was then returned to its container in the controlled environment room.
Furthermore, host condition was additionally controlled as the colonies were kept in exactly the same conditions in the same controlled environment room.
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Mice were housed in controlled environment rooms (72°F, ~40% humidity, 12 h/12 h in-phase light/dark cycles at 6 am/6 pm) in ventilated racks (Thoren; Hazelton, PA).
Plants were grown in controlled environment rooms (CERs) with an 8 hr/16 hr dark/light cycle, a constant 80% relative humidity and a light intensity of approximately 350 μmol m-2 s-1.
Responses to vernalization from 126 Arabidopsis accessions were obtained from published data [ 22] and analyzed by subtracting days to flowering with vernalization from days to flowering without vernalization (plants grown in controlled environment rooms with 16 hour light).
A. thaliana (Columbia ecotype) and B.rapa plants were grown in Phoenix Biosystem controlled environment rooms at 21°C under metal halide lights that provided a level of PAR (photosynthetic active radiation) of 110 μmol of photos/m/s.
was sown and grown as described [ 23] in controlled environment rooms with days of 8 h at 21°C, 150 μmol m-2 s-1, and nights of 16 h at 19°C.
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