You need to know the lipid membrane environment, their conformation, their dynamics, the feel for it," she says.
The stereochemical environment, the conformation of the backbone and side chains was analysed using PROCHECK [35].
Not only are the samples homogeneous and well defined in terms of components and their concentrations, the substrates are in a uniform environment and conformation inside the lumen.
Gramicidin is a linear pentadecapeptide antibiotic that adopts a wide range of environment dependent conformations.
The apparent influence of the protein environment on the conformation of the methoxy groups in each particular case suggests that the role of the methoxy conformations in the Qi site of the bc1 complex could be better understood from experiments with Qi site mutants.
Since membrane environment influences the conformation of the MPER29, we generated gp41-MinTT-based proteoliposomes using POPC and lipids overrepresented in the viral membrane-like environment, such as CHOL or SM31,45,46.
The effects of alterations in the environment on the conformation of melimine were investigated using circular dichroism and fluorescence spectra in membrane-mimetic solvents.
In particular, approaches based on the equalization of molecular electronegativity [22, 23, 28 35] are of interest because they are sensitive to both the chemical environment and molecular conformation.
Thus, we propose the following molecular-cascade: elevated oxidative environment affects p53 conformation towards an unfolded structure.
H N HSQC NMR spectroscopy is widely employed to monitor protein folding characteristics and solution behavior, and the spectra are often termed the "fingerprint" of the protein for their sensitivity to any change in conformation, environment, or interaction.
