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Cells were disrupted by sonication, unbroken cells removed by low-speed (2,000 × g) centrifugation and, finally cell envelopes collected by ultracentrifugation (40,000 × g) for 60 min at 4°C.
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Also, to allow for confidentiality, respondents were asked to put their completed questionnaire in a sealed envelope and the envelopes are collected by the research coordinator and then returned directly to the researchers.
The sealed envelopes were collected by the independent researchers and transported to the School of Public Health, Curtin University of Technology, Perth, Australia.
At the conclusion of the study, all catalog envelopes were collected and a total of 66 mind maps were available for analysis.
Starting with the N Tth updates, the sample data F X (m) of the WB audio feature, the hidden states S hidden(m), and the sample data F Y (m) of the HF spectral envelope are collected for establishing the state collection matrix B and the expectation output matrix Q.
Completed answer sheets were immediately put in an opaque envelope and collected by research assistants.
In order to have an estimate of the current knowledge of protein complexes and PPIs occurring at the cell-envelope, we collected high-confidence PPIs deposited in three public databases, namely DIP (Salwinski et al., 2004), BIND (Bader et al., 2003) and IntAct (Hermjakob et al., 2004) (Table 3).
As co-founder and CEO Brett Kopf explained, most schools still rely on cash and envelopes to collect money for an average of 12 activities per student each year.
As co-founder and CEO Brett Kopf explained, most schools still rely on cash and envelopes to collect money for an average of 12 activities per student each year.
All envelopes will be collected by the site monitor at the end of the trial.
Make sure they have an envelope to collect completed surveys after the lecture.
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