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Antibodies against conformational envelope were detected by an immunoflourescent staining assay for Vero cells expressing envelope.
Antibodies bound to envelope were detected by an anti-human IgG fluorescent dye (Alexa Fluor 594 goat anti-human IgG, Invitrogen, Carlsbad, CA).
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Interestingly, envelope escape variants were detected due to G145R, D144DE and P142LP mutations.
The LFEs were detected using the Envelope Correlation Method, and the hypocenter locations were established using the arrival time difference of the envelope.
Upon cell fractionation, the polysaccharides were detected in the envelope and cytosol of the wzm and wa** mutants, respectively.
Presence of HA-Vpr, Vpx or HA-Vpr/Vpx proteins in the supernatant fraction indicated successful packaging into vector particles, as in the absence of the Gag-Pol and the envelope constructs none of those proteins were detected.
However, two HIV-1 positive samples, Cal21 and Cal22 were missed by these proteins but were detected by another single antigen construct carrying cysteine in envelope (B6Fabenv1C).
All three were detected even without trypsin treatment, suggesting a loose association with the cell envelope.
After ignition, the formation of an envelope flame is detected.
When a bridge signature falls outside the baseline bridge signature envelope, damage is detected.
In addition, each envelope protein was detected to interact with more than one capsid protein.
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CEO of Professional Science Editing for Scientists @ prosciediting.com