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These results ruled out the possibility of a bacterial envelope defect leading to an non-specific release of all tested drugs, but favored an alteration of the drug efflux mechanism of the tolC mutant.
Sensitivity to many drugs would be more apparent if the envelope defect was strong.
Cell structure proteins (envelope proteins) were biggest part of the total protein content, and inhibition of protein synthesis might cause cell envelope defect.
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In some instances, it may be possible to close soft tissue envelope defects by preserving length and intentionally creating a deformity of the limb.
Nuclear envelope defects were observed in diploid imaginal disc tissue.
Drosophila lacking A-type lamin show nuclear envelope defects similar to those observed with human laminopathies.
The existence of a connection between the nucleus and cytoplasm is consistent with the fact that Lamin C nulls show muscle cell nuclear envelope defects (Figs. 4 and 5).
The fact that we did not isolate other mutants with cell envelope defects such as those from the Tat or Tol-Pal systems suggests that inability of the smpA mutant to grow on LIM media may not be directly related to its outer membrane defect.
(C ) CpoB-associated envelope defects visualized by fluorescence microscopy using periplasmic mCherry.
Cells lacking CpoB exhibited envelope defects that were severely exacerbated in the absence of LpoA and alleviated by PBP1B overexpression.
In C. elegans, mel-28 disruption leads to severe nuclear envelope defects (Fernandez and Piano 2006; Galy et al. 2006).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com